Bahareh Davarnia; Ali Motamedzadegan; Gholamhasan Asadi; Abdolmohammad Abedian Kenari; Mahmoudreza Ovissipour
Abstract
Enzymatic hydrolysis of Yellowfin tuna visceral protein with Neutrase has been carried out by response surface methodology using factorial design. Peptide chain length was estimated as the response surface to the hydrolysis conditions (enzyme activity, reaction temperature, and reaction time). The tuna ...
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Enzymatic hydrolysis of Yellowfin tuna visceral protein with Neutrase has been carried out by response surface methodology using factorial design. Peptide chain length was estimated as the response surface to the hydrolysis conditions (enzyme activity, reaction temperature, and reaction time). The tuna visceral protein hydrolysate had relatively high protein (74.56%) and low lipid content (1.86%). R2 of 0.85for the mathematical model indicated that 85% of the variability within the range of values studied could be explained by the model. Enzyme activity of AU/kg protein, temperature of 50°C, and hydrolysis time of min were the optimal conditions of hydrolysis. The electrophoresis pattern of the Yellow tuna hydrolysate showed no peptides size bigger than 10 KDa. The chemical score of the hydrolysate indicated that it fulfils the nutritional requirements of children with age 10-12 except Lysine and Methionine. Lysine and Methionine were the first and the second limiting amino acids, respectively and in case of children with age 2-5 lysine was predominant amino acid in the hydrolysates. It could be concluded that by application of enzymatic hydrolysis of Yellowfin tuna viscera protein, the properties of protein hydrolysate was improved.
Maryam Modanlow; Gholamreza Rafiee; Ali Motamedzadegan; Sohrab Moeini; Alireza Mirvaghefi; Mahmoudreza Ovissipour
Abstract
In present paper the optimum extraction condition of protein of Hydrolysate from the viscera of yellow fin tuna (Thunus albacores) were studied. Enzyme hydrolysis has been performed at enzyme to substrate ratio: 1.3-3.7 mg/gr.protein , time of reaction: 1-10 hours, temperature: 30-44 ºC and using RSM ...
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In present paper the optimum extraction condition of protein of Hydrolysate from the viscera of yellow fin tuna (Thunus albacores) were studied. Enzyme hydrolysis has been performed at enzyme to substrate ratio: 1.3-3.7 mg/gr.protein , time of reaction: 1-10 hours, temperature: 30-44 ºC and using RSM method and central composite design(CCD) with 4 repetitions at central point and five levels of each treatment. Results have been compared with average at statistical level where α = 0.05. According to results,the protein recovery increases with an increase in enzyme activity from 1.3 to 2.5 mg When enzyme activity increases to 3.35 mg, the protein recovery reaches a constant level and then decreases with an extra increase of enzyme amount to 3.7 mg.The rate of protein recovery increases, With an increase in temperature from 30 ºC to 37 ºC,And then the enhancement of recovery rate decreases with more increase of temperature from 37ºC to 42ºC, However it will remain constant for temperatures beyond 42ºC.The protein recovery decreases With increasing the hydrolysis time between 60 to 138 min. and it reaches a stationary phase somehow during 138 to 330 min.In optimum point of hydrolysis, protein recovery was 75%. This condition were observed in enzyme ratio of 2.41 mg ,Temperature of 38.75ºC,and hydrolysis time of 570 min. Results showed that protein recovery is mainly influenced by ratio of enzyme to substrate rate and temperature ,while time of hydrolysis should be considered as a function of temperature and enzymatic ratio.
Keywords: Protein hydrolysate, Protein recovery, Optimization, Waste of yellow fine tuna, Trypsin
